Table 2.

Bioassay of native and in vitro-generated recombinant PrP^Sc molecules in normal C57BL mice

Seed (strain)	Inoculum	Dilution	n/n0	IP (days)*
OSU	Input recPrPSc	100	5/5	173 ± 1
OSU	Cofactor recPrPSc	10−1	7/7	356 ± 12
OSU	Synthetic PE recPrPSc	10−1	8/8	381 ± 11
OSU	Serial Passage- Synthetic PE recPrPSc	1% wt/vol	8/8	175 ± 4
OSU	Mock sPMCA control	10−1	0/4	>570
Me7	Input brain homogenate	10−1	7/7	158 ± 7
Me7	Cofactor recPrPSc	10−1	8/8	396 ± 20
Me7	Synthetic PE recPrPSc	10−1	6/6	417 ± 21
Me7	Serial Passage- Synthetic PE recPrPSc	1% wt/vol	8/8	166 ± 3
Me7	Mock sPMCA control	10−1	0/4	>510
301C	Input brain homogenate	10−1	7/7	182 ± 4
301C	Cofactor recPrPSc	10−1	8/8	401 ± 11
301C	Synthetic PE recPrPSc	10−1	6/7	360 ± 9†
301C	Serial Passage- Synthetic PE recPrPSc	1% wt/vol	8/8	173 ± 3
301C	Mock sPMCA control	10−1	0/4	>510
None	Cofactor mixture	10−1	0/4	>570
None	Synthetic PE mixture	10−1	0/4	>570

*Incubation period (IP) of scrapie sick animals, mean ± SE.

^†Ongoing experiment. n/n₀ = number of animals diagnosed with prion disease/number of animals inoculated.