Fig. 4.

Contribution of MER20 vs. MER39 to activity of ape dPRL promoter. (A) Activity of chimeric MER20–MER39 promoters from orangutan and OWM. Structure of each promoter–reporter construct tested is indicated above activity level: light gray indicates region is derived from orangutan and dark gray from OWM; black represents the reporter construct. (B) Core ETS1 sequence in apes, and orthologous positions in other primates and rodents. (C and D) Activity of mutated orangutan (C) and OWM (D) promoters (Mut1–7) with additional ETS1 site mutated (Mut1–7.ETS). Note that the ETS1 site and sites 3–7 (Fig. 3A) are in MER39. ***P < 0.001 (t test) vs. activity of indicated construct. Significant differences shown in Fig. 3 are not repeated.