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. 2012 Jun 25;109(28):11425–11430. doi: 10.1073/pnas.1208557109

Fig. 4.

Fig. 4.

ROS4 does not regulate the expression of RD29A-LUC or of the endogenous RD29A gene. (A) Luminescence images of different mutants. Ten-day-old seedlings were treated with 300 mM NaCl for 3.5 h before luminescence images were obtained. (B) Quantitative RT-PCR analyses of LUC transcripts in different mutants. Three independent experiments (each with three biological replicates) were done with similar results. Error bars refer to standard error (n = 3). (C) DNA methylation analyses of the transgene RD29A (tRD29A) and the endogenous RD29A (eRD29A) promoter by bisulfite sequencing in the wild type, ros1-1, ros4, nrpd1-8, and nrpe1-14. (D) The expression of the endogenous RD29A gene in the wild type, ros1-1, ros4, nrpd1-8, and nrpe1-14 under 300 mM NaCl treatment. The stress-inducible gene COR47 was used as a control for effective stress treatments.