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. 2012 Jun 25;109(28):11425–11430. doi: 10.1073/pnas.1208557109

Fig. 5.

Fig. 5.

ROS4 is important for the ddm1-promoted expression of endogenous genes. (A) The expression of six selected genes as determined by qRT-PCR in the wild type, ros4, ddm1, and ros4 ddm1. At least three independent experiments (each with three biological replicates) were done with similar results. (B) ROS4-Myc binds to the 35S promoter and the promoters of four endogenous genes as indicated by ChIP assay. The ChIP assay was performed on 7-d-old seedlings of the ros4 mutant complemented by the ROS4 promoter::ROS4-Myc, the wild type, and a transgenic plant expressing BAK1-Myc. At least three independent experiments (each with three biological replicates) were done with similar results. (C) H3K18ac, H3K23ac, and H3K4me2 levels in the wild type, ros4, and ros1-1. The ChIP assay used 7-d-old seedlings and H3K18ac, H3K23ac, or H3K4me3 antibodies. No antibody was used as a negative control. In A–C, error bars refer to standard error (n = 3).