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(A) Diagram depicting the URS1 site in the ATG8 promoter. (B) The Ume6-Sin3-Rpd3 complex represses Atg8 expression. Wild-type (BY4742), ume6Δ, sin3Δ, and rpd3Δ yeast cells were grown in rich medium to midlog phase. Protein extracts from cells were prepared and subjected to immunoblotting with anti-Atg8 and anti-Pgk1 antiserum (the latter as a loading control).
