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. 2012 Jun 25;109(28):11437–11442. doi: 10.1073/pnas.1119173109

Fig. 2.

Fig. 2.

ebs3-1 is a weak suppressor of the bri1-9 mutant. (A–C) Images of 3-wk-old soil-grown plants (A), 5-d-old etiolated seedlings (B), and 7-wk-old soil-grown mature plants (C) of WT, bri1-9, and ebs3-1 bri1-9. [Scale bars: 1 cm (A and B) and 10 cm (C).] (D) Quantitative analysis of BR sensitivity. Root length of 10-d-old seedlings grown on BL-containing medium were measured and presented as the relative value of average root length of BL-treated seedlings to that of mock-treated seedlings. Each data point represents the average of ∼40 seedlings of duplicated experiments. Error bars denote SE. (E) Immunoblot analysis of BL-induced BES1 dephosphorylation. Total proteins were extracted in 2× SDS buffer from 2-wk-old seedlings treated with or without 1 μM BL for 1 h, separated by SDS/PAGE, and analyzed by immunoblot using an anti-BES1 antibody (27). Star indicates a nonspecific band for loading control.