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. 2012 Jun 25;109(28):11360–11365. doi: 10.1073/pnas.1209293109

Fig. 4.

Fig. 4.

Overexpression of p11 in NAc cholinergic interneurons of constitutive p11KO mice restores normal behavior. (A) Schematic of the virus used in these experiments (see text). Constitutive p11 KO mice were bred with ChAT-CRE mice to generate p11 KOs that expressed CRE in ChAT neurons. (B) Immunohistochemical detection of RFP (red), ChAT (blue), and p11 (green) in the NAC of a p11 KO mouse expressing (Upper) or not expressing (Lower) CRE in ChAT neurons. Arrows indicate ChAT-positive cholinergic interneurons overexpressing (Upper) or not over-expressing (Lower) p11. (C–E) Control virus (aav-YFP) or p11 overexpressing virus (aav-p11) was injected to the NAc of CRE-positive WT or p11 KO mice before behavioral testing in the TST [F(1,32) = 6.384, P < 0.05] (C), the sucrose preference test (F(1,32) = 5.681, P < 0.05) (D), or the open field test [main effect AAV: F(1,37) = 3.505, n.s.; main effect genotype: F(1,37) = 0.2654, n.s.] (E). All data are presented as means ± SEM. Significant effects of genotype (#P < 0.05) or p11 overexpression (*P < 0.05, **P < 0.01) are noted. (F–H) CRE-positive constitutive p11KO mice were injected with aav-p11 or aav-YFP into the dorsal striatum (CPU). No effect of p11 overexpression was observed in sucrose preference (main effect virus: F(1,26) = 0.3801, n.s.; main effect genotype: F(1,26) = 1.136, n.s.; interaction virus × genotype: F(1,26) = 0.1272, n.s.) (F), tail suspension test immobility (main effect virus: F(1,25) = 1.994, n.s.; main effect genotype F(1,25) = 1.521, n.s.; Interaction virus × genotype: F(1,25) = 3.874, n.s.) (G), or locomotor activity (main effect virus: F(1,26) ≤0.001, n.s.; main effect genotype: F(1,26) =1.244, n.s.; interaction virus × genotype: F(1,26) = 1.022, n.s.) (H). All data are presented as means ± SEM.