Panels (a) and (b) show the ribbon diagram of the
protein models of ubiquitin and the RNA-binding domain of the nonstructural
protein 1 of the influenza A virus, respectively; these models were
obtained46 after one
round of Simulated Annealing Refinement (SAR) starting from the deposited PDB
structures of 1UBQ and 1AIL, and represented by the orange bars in
(c) and (d); these two panels also show the bar
diagram of the rmsd between computed and observed
13Cα chemical shifts, as cyan-filled bars, for
the generated ensemble of conformations, generated from the SAR PDB models and,
at the same time, showing R and Rfree factors similar to those of the
deposited X-ray structure;46
the black solid horizontal lines represent the ca-rmsd for each
ensemble (2.36 ppm and 1.92 ppm for UBQ and AIL, respectively).