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. Author manuscript; available in PMC: 2013 Mar 1.
Published in final edited form as: J Inorg Biochem. 2011 Nov 27;108:105–111. doi: 10.1016/j.jinorgbio.2011.11.005

Fig. 5.

Fig. 5

Proposed reaction mechanism of AF with TGR. The mechanism of inhibition of AF towards Sec-containing flavoreductases is envisaged as a series of displacement reactions of the ligands of the metal, presumably occurring via a transient three-coordinate state. The acetoxythioglucose (tagl) is lost upon nucleophilic attack of the Sec, accordingly to Shaw [32]. The metal could be either be trapped by the adjacent Cys loosing the P(Et)3 or be transported to the more exposed Cys of the FAD active site. Both of these mechanisms may account for the arrangement of Au observed in the crystal structure of SmTGR in complex with AF (Fig. 3 panel A; pdb code: 3h4k [11]).