Figure 2. Maturation of the tight junction occurs as the nephron expresses markers of epithelium.

A) A diagrammatic representation of the columnar epithelium of the proximal tubule. B) Western blot of early (0 day) and late (7 day) nephron cultures. Intercellular junction proteins specific to the tight and adherens junctions (Including the nephron specific KSP-Cadherin) denote the mesenchymal to epithelial transition during development of the nephron. C) Western blot showing detergent extraction of nephron cultures from 3 to 5 days. GRP94 was used as a control for extraction.