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. 2012 Jul 13;7(7):e40763. doi: 10.1371/journal.pone.0040763

Figure 6. TLR2Ab suppressed the expression of endogenous TLR2 agonists and interaction of TLR2 with HMGB1.

Figure 6

(A) Expression of HMGB1 and Hsp70 were reduced in TLR2Ab-treated mice, but not in TLR4Ab group. (B) TLR2Ab and TLR4Ab suppressed its downstream signaling pathway. Data are shown as mean ± SEM (n = 5/group). (C) TLR2Ab inhibited the interaction of TLR2 with HMGB1 as indicated by co-immunoprecipitation and western blot. Data are representatives of two experiments with identical results. (D) TLR2Ab decreased the co-localization of TLR2 and HMGB1 as shown by confocal microscopy. Data are representatives of two experiments with identical results. #p<0.05, ##p<0.01, ###p<0.001 versus saline-treated group; *p<0.05, **p<0.01, ***p<0.001 versus Dox+IgG-treated group.