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. 2012 Jul 13;7(7):e40594. doi: 10.1371/journal.pone.0040594

Figure 1. Protein 14-3-3.

Figure 1

ζ expression increases with oncogenic transformation in prostate cancer cells. (A) Murine TRAMP (TR-C2D, TR-C2 and TR-C2N), human hormone responsive LNCaP as well as hormone insensitive LNCaP C4-2 and PC3 prostate cancer cell lysates were subjected for western blot comparative analysis for 14-3-3ζ expression. (B) Quantification of the above data by band densitometry analysis showing increased expression of 14-3-3ζ in prostate cancer cells compared to non-tumorigenic murine TRC2D cells. (C-E) PC3 cells were transfected with control vector, WT-14-3-3ζ and DM-14-3-3ζ and were subjected to viability (Trypan blue assay), Proliferation (MTT assay) and colony formation assay, respectively. (*p<0.001; Δp<0.01; #p<0.05; n = 3).