Figure 3. Protein 14-3-3ζ expression and dimerization leads to activation of Rac1-p21 activated kinase (Pak1) pathway in prostate cancer cells.

(A) WT-14-3-3ζ, DM-14-3-3ζ, CA-Rac1 (L⁶¹) and DN-Rac1 (N¹⁷) expression plasmids and vector, alone or in combinations, were transfected to PC3 cells and cell lysates were subjected to Western analysis of phosphorylated Pak1. (B) Quantification of the above data by band densitometry analysis. Bars depict Pak1 activity as measured by the levels of phosphorylation. (*p<0.001; Δp<0.01; #p<0.05; n = 3).