Figure 4. RNAi targeting chitin deacetylases affect developmental timing.

dsRNA targeting both putative polysaccharide deacetylases F48E3.8 and C54G7.3 was introduced to the wild-type N2 strain (A,B) by feeding. dsRNA targeting the C54G7.3 gene alone was introduced to the RNAi-hypersensitive strain NL2099 (C,D) by feeding. Single L3 larvae were allowed to proceed through development while feeding before progeny were scored at 48 hours (A,C) and 72 hours (B,D). RNAi targeting both genes results in a delay in wild-type worm development that can also be observed when C54G7.3 is targeted through RNAi in a strain hypersensitive to exogenous RNAi.