Figure 1. Setup of the clinostat model system.

The clinostat is equipped with computerized temperature and motor controls. Cells were initially seeded on coverslips, and then the coverslips were inserted into the fixtures of the chambers. After that, the chambers were filled completely with F-12K with 10% FBS (filling chambers with complete medium, taking care to avoid air bubbles). These chambers underwent rotation at 30 rpm around the vertical axis for 24 h. NG controls were static control cultures kept in the same room as the clinostat.