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. 2012 Jul 13;7(7):e40365. doi: 10.1371/journal.pone.0040365

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Shi et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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HUVEC-C were cultured under a static condition or exposed to clinorotation for 24 h. The eNOS mRNA levels were determined by RT-PCR in both groups, as described in Materials and Methods. Amplification of cDNA was performed in parallel samples using human GAPDH primers. Data are presented for expression of eNOS as a percentage change in band density normalized to GAPDH samples, and are shown as mean ± SD from 4 independent experiments. Results show that simulated microgravity significantly increase the eNOS mRNA level in HUVEC-C. ^* P<0.05 versus control.