Figure 3. Evaluation of STM1485 expression in epithelial cells (INT-407, Caco-2 and HeLa). STM1485 expression was studied using (A) RT-PCR assay. (B) Immunoblot method using the STM1485::6xHis tagged strain. Bacteria were isolated from the infected epithelial cells at 6th h post infection. Bacteria grown in DMEM at 37°C under 5% CO₂ were served as control to determine expression outside host cells. Numbers below the gels indicate the values of densitometric image analysis using Multi Guage software. Results are representative of two independent experiments.