Figure 6. Effect of phagosomal pH neutralization on intracellular replication of ΔSTM1485 strain. RAW264.7 cells were pretreated with (A) bafilomycin A1 (50 nM), (B) Folimycin (100 nM) and (C) NH₄Cl (100 mM) followed by infection as described in materials and methods. Infected cells were lysed at 2 h and 16 h post infection and bacterial load was shown as fold increase in CFU from 2 h to 16 h. Graphs are representative of three independent experiments with similar results. Statistical significance was defined as follows (*p < 0.05, **p < 0.005) (Student's t-test).