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. Author manuscript; available in PMC: 2013 Aug 1.
Published in final edited form as: Immunogenetics. 2012 Jun 9;64(8):647–652. doi: 10.1007/s00251-012-0626-0

Figure 2.

Figure 2

Phylogenetic tree based on nucleotide alignments of VpreB1, 2, and 3 along with Vλ and Vκ genes from the species indicated. The opossum VpreB3 is bolded and boxed. Analyses were performed on nucleotide alignments using the neighbor-joining and minimal evolution methods in MEGA4 with similar results; the minimal evolution tree is shown (Tamura et al. 2007). Amino acid translations were first aligned to establish gap position and then converted back to nucleotide using the BioEdit program (Hall 1994). The GenBank accession numbers of the sequences used in the phylogenetic analysis were: Opossum VpreB3, JN863116; Human VpreB1, CR456609; Human VpreB3, NM_013378; Human VL5b, BAA20017; Human VL4c, CAA80218; Human VL9a, CAP74528; Human VL1a, BAA20004; Human VL6a, AAB33217; Chimpanzee VpreB1, NW_003458635; Chimpanzee VpreB3, NW_003458643; Rhesus Monkey VL5, AM056012; Mouse VpreB2, BC141459; Mouse VpreB1, NM_016982; Mouse VpreB3, NM_009514; Mouse VLX, AAA39169; Rat VpreB2, NM_001134788; Rat VpreB1, NM_001108845; Rat VK, EDL82784; Hamster VK, AAA82732; Rabbit VpreB2, AY351268; Rabbit VpreB3, XM_002724010; Rabbit VpreB1, AY351269; Rabbit VL2, PS0055; Rabbit VL3, PS0056; Sheep VK, S33161; Cow VpreB3, NW_003104461; Cow VpreB1, NW_003104461; Horse VK1, CAA53283; Pig VpreB3, NW_003611976; Pig VpreB1, NW_003611976; Elephant 490, NW_003573490 (1148141-1148468); Elephant 490b, NW_003573490 (3338560-3338236); Elephant 560, NW_003573560; Dog VpreB1, NC_0066083; Dog Vi930, XM_003639930; Dog Vi107, NW_003726107; Opossum VK, XP_003339882; Opossum VKII, XP_003339837; Possum VKI, AY074425; Possum VLIV, AAM09967; Opossum VLI, AF049774; Echidna VL167, AAM76525; Platypus VL1, AAO16067; Platypus VL97, AAO16074; Chicken VpreB3, XM_415223; Zebra finch VpreB3, NW_002197395; Turkey VpreB3, NW_003436164; Chicken VL1, I51216.