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. Author manuscript; available in PMC: 2013 Feb 1.
Published in final edited form as: Kidney Int. 2012 Apr 11;82(3):278–291. doi: 10.1038/ki.2012.102

Figure 5. HO-1 expression is induced at higher levels after rhabdomyolysis in hHO-1 BAC mice.

Figure 5

Rhabdomyolysis was induced by injecting 50% glycerol (7.5 ml/kg body weight) in HO-1+/+ and hHO-1 BAC mice (A and B, respectively) (n=9/group). Kidneys from HO-1+/+ and hHO-1 BAC mice were collected and total RNA was isolated. At 8 hours post glycerol administration HO-1 was induced ~30 fold in HO-1+/+ (panel A) kidneys, while the hHO-1 BAC kidneys revealed ~14 fold HO-1 induction (panel B) *p<0.01. (C) Microsomal fractions from kidneys of HO-1+/+ and hHO-1 BAC mice after injection of saline (S) as a control and 50% glycerol (G) for 16 h were analyzed for HO-1 protein expression using Western analysis. Note that normalization of the band intensity to corresponding actin levels shows no significant difference between glycerol treated HO-1+/+ and hHO-1 BAC saline treated mice. (D) Kidneys of HO-1+/+ and hHO-1 BAC mice after injection of saline (S) and glycerol (G) were collected and HO enzyme activity was measured at 16 h after glycerol administration as described in the Methods. (n=3/group) *p<0.05 vs. HO-1+/+ (E) Survival rate was monitored after induction of rhabdomyolysis in HO-1+/+, HO-1−/−, and hHO-1 BAC mice (n=10/group). Experiments were ceased 10 days after injection. (F) Serum creatinine was measured in HO-1+/+, HO-1−/−, and hHO-1 BAC mice after indicated days after induction of rhabdomyolysis.