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. Author manuscript; available in PMC: 2013 Aug 1.
Published in final edited form as: Traffic. 2012 May 28;13(8):1170–1185. doi: 10.1111/j.1600-0854.2012.01376.x

Figure 9. N-glycosylation of Crumbs3a is not critical for trafficking to or stability at the apical cell surface.

Figure 9

A) Lysates from cells expressing wild type SNAP-Crb3a (WT) or SNAP-Crb3a-N36D (N36D) immunoblotted with indicated antibodies. B) Comparison of the half-lives of cell surface WT or N36D SNAP-Crb3a. Cells were labeled in-culture with BG-682, incubated for indicated time points, lysed and immunoblotted with anti-Crb3a antibody. B) Graph showing residual fluorescence of cell surface labeled SNAP-Crb3a normalized to total SNAP-Crb3a signal and expressed relative to the 0 time point. The experiment was performed twice in duplicate; a representative immunoblot is shown. The graph shows averaged experimental time points with standard deviations.