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. Author manuscript; available in PMC: 2013 Aug 1.
Published in final edited form as: Lung Cancer. 2012 May 6;77(2):265–271. doi: 10.1016/j.lungcan.2012.04.010

Fig. 2.

Fig. 2

Protein expression of vitamin D receptor (VDR) and the effect of 1,25-dihydroxyvitamin D3 (1,25-D3) in human lung cancer cell lines. A, immunoblot analysis of VDR protein in high (SKLU-1) and low (A549) VDR mRNA expressing lung adenocarcinoma (AC) cell lines.β-actin was used as a loading control. The protein expression of VDR was significantly higher in SKLU-1 cells. B, the effect of 1,25-D3 on cell proliferation in A549 and SKLU-1 cell lines by WST-1 assay. SKLU-1 demonstrated more marked decrease in cell proliferation in response to 1,25-D3 compared to A549 at both days 3 and 6. Protein expression and cell proliferation experiments were performed at least three times independently.