Figure 4. Co-immunoprecipitation of Rep14F and WT-M Receptors from Co-transfected HEK 293 Cells.

The ability of WT-M to co-immunoprecipitate with Rep14F was measured by immunoprecipitation assays as in Figure 3. A,B: Immunoprecipitation with α-FLAG affinity resin followed by blotting with α-FLAG (A) or α-Myc (B) antibodies. C,D:α-Myc immunoprecipitation and subsequent immunoblot analysis of Rep14F and WT-M. The blots shown are representative of 3 replicate experiments.