Fig. 3. Pro-oxidants Increase KLF6_Full.

CYP2E1-expressing cells were treated in duplicate with 15 μM menadione, 100 μM paraquat or 100 μM β-naphtoflavone for 6 h (A) or in a time-course study up to 24 h with 15 μM menadione (B) and KLF6_Full expression was evaluated by Northern blot analysis. A value of 1 was given to the untreated cells in (A) and to the first time-point to show any signal in (B). Control and CYP2E1-expressing cells were treated with 30 μM AA, 20 μM H₂O₂, 15 μM menadione or the combination of AA plus H₂O₂ and AA plus menadione for 6 h and KLF6_Full expression was assessed by Northern blot analysis. The quantification of the signal indicated under the blots is the average of N=2. The signal for the untreated controls was assigned a value of 1 (C).