Fig. 8. KLF6_V1 Ablation Up-Regulates TNFα in the presence of AA.

RAW 246.7 macrophages were transfected with the -982-TNFα promoter. Then, a co-transfection was carried out with control, KLF6_Full or KLF6_V1 siRNAs. In some experiments, cells were treated with 30 μM AA for 3 h. Luc activity after 48 hours of transfection was corrected by transfection efficiency and normalized with Renilla-Luc (A). Secreted TNFα was measured in the respective supernatants by ELISA (B). Results are expressed as mean ± SEM (N=6; ^■P<0.05, ^{■ ■}P<0.01 and ^{■ ■ ■}P<0.001 for any siRNA vs. control and •P<0.05, ••P<0.01 and •••P< 0.0 01 f or A A-treated vs. control).