Table 1.
mRNA | Forward primer | Reverse primer | Amplicon size |
---|---|---|---|
KLF6Fu ll | 5′-CGGACGCACACAGGAGAAAA-3′ Exon 2-Exon 3 (nt 763–782) | 5′-CGGTGTGCTTTCGGAAGTG-3′ Exon 3 | 103 bp |
KLF6_V 1 | 5′-CCTCGCCAGGGAAGGAGAA-3′ Exon 2-Exon 3 (nt 509–780) | 5′-CGGTGTGCTTTCGGAAGTG-3′ Exon 3 | 104 bp |
KLF6_V 2 | 5′-GTCGGGGAAGCCAGAGAA-3′ Exon 2-Exon 3 (nt 607–780) | 5′-CGGTGTGCTTTCGGAAGTG-3′ Exon 3 | 103 bp |
GAPDH | 5′-CAATGACCCCTTCATTGACC-3′ | 5′-GATCTCGCTCCTGGAAGATG-3′ | 113 bp |