(A) Increased optic nerve volume (white arrow) and abnormal cell clusters (black arrow) were observed in optic gliomas from 3-month-old Nf1+/− GFAP mice. (B) The potential cellular origins (lv-SVZ and TVZ) of optic gliomas in Nf1 mutant mice are illustrated. LV: lateral ventricle, red: lateral geniculate nucleus, green: optic tract, TV: third ventricle, yellow: optic chiasm. (C) Increased TVZ neurosphere proliferation was seen following Nf1 loss, with little effect on lv-SVZ NSCs. (D) 3-fold more Olig2+ cells were found in the TVZ of Nf1BLBP mice (p=0.0004), but not in the lv-SVZ (p=0.0836), compared to controls. (E) Increased numbers of GFAP+ cells were found in the TVZ, but not in the lv-SVZ, of Nf1BLBP mouse compared to controls. Values denote the mean ± SEM. See also Figure S2.