Table 2.

Medium conditions for bovine embryonic stem cell-like cell culture

Medium type	Fundamental ingredient	Cytokine concentrations
Medium I	Fundamental ingredient	20 ng/mL LIF + 10 ng/mL bFGF
Medium II	Fundamental ingredient	20 ng/mL LIF + 10 ng/mL SCF + 10 ng/mL bFGF
Medium III	Fundamental ingredient	10 ng/mL SCF + 10 ng/mL bFGF

Fundamental ingredient which consisted of 90% DMEM/F12 (Gibco) supplemented with 10% fetal bovine serum (Gibco), 0.1 mM β-mercaptoethanol (Chemicon) , 0.1 mM nonessential amino acids, 100 IU/ml penicillin, 0.05 mg/ml streptomycin