FIG. 3.

Detection of pluripotency of hESCs after five passages on PET and PE membranes. (A) Cells were stained with antibodies against OCT4 and SSEA4 marker proteins after five passages. Cells were also labeled with diaminophenylindole (DAPI) in order to localize the nucleus. Stained cells were observed under a fluorescence microscope (Olympus IX 71) equipped with a charge-coupled device (CCD) camera. Scar bar: 100 μm. (B) Gene marker of hESCs and markers of the three germ layers were detected in cells grown on membrane substrates using quantitative real-time–polymerase chain reaction (qRT-PCR) analysis. Control: hESC line H9 cultured on a TCP substrate in undifferentiated state. Color images available online at www.liebertonline.com/tea