ECM treatment results in the accumulation of a heterogeneous population of cells at the site of amputation. After digit amputation and treatment with either ECM degradation products or control, the accumulation of cells at the site of amputation (Fig. 2.) was microdissected and dissociated for further analysis. Flow cytometric analysis showed that subsets of cells expressed markers Sca1, CD146, Sox2, Lineage, CD11b, and F4/80.