FIG. 5.

Sox2+ cell accumulation requires bone injury and is located lateral to the amputated bone. (A) Immunolabeling of histologic sections of amputated digits showed that the majority of Sox2+ cells present at the site of amputation after treatment with ECM degradation products were located lateral to the amputated P2 bone, consistent with a periosteal location. (B) After digit amputation proximal to P2 bone at the joint such that no bone injury was induced, the accumulation of Sox2+ cells at the site of amputation after ECM degradation products was decreased. *p<0.05. **p<0.01. Error bars are mean±SEM (n=4 for each group).