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. 2012 May 31;18(13-14):1479–1489. doi: 10.1089/ten.tea.2011.0325

FIG. 1.

FIG. 1.

Outline of the experimental protocol. MSC-CM was collected after cultivation of hMSCs for 48 h in serum-free medium. A rat calvarial bone defect of 5 mm in diameter was produced in each parietal bone. The effect of MSC-CM on cell migration, proliferation, and gene expression in vitro, and of implanted MSC-CM on bone regeneration in vivo were then analyzed as shown. hMSCs, human mesenchymal stem cells; CM, conditioned media; rMSCs, rat MSCs; RT-PCR, reverse transcriptase-polymerase chain reaction.