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. 2012 May 31;18(13-14):1479–1489. doi: 10.1089/ten.tea.2011.0325

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Copyright 2012, Mary Ann Liebert, Inc.

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FIG. 2. — Effect of MSC-CM on the migration and proliferation of rMSCs. (A) Transwell migration assay. The migration of rMSCs cultured in MSC-CM was enhanced compared with that of rMSCs cultured in DMEM(−). (B) BrdU cell proliferation assay. Proliferation of rMSCs was determined as the percentage of cells that incorporated BrdU. The proliferation of rMSCs was also enhanced when cultured with MSC-CM compared with culture in DMEM(−). Cells cultured in DMEM-30%FBS were used as a positive control for both (A) and (B). Asterisks indicate a significant difference between the indicated groups (*p<0.05). FBS, fetal bovine serum; BrdU, bromodeoxyuridine; rMSCs, rat MSCs; DMEM, Dulbecco's modified Eagle's medium.