Table 1.
Origin of the SSR primers, PCR protocols and annealing temperatures and the results of their amplification
| Species | Reference | PCR protocola | Annealing temperature (°C) | Total tested | No amplification | Monomorphic | Not scorable |
|---|---|---|---|---|---|---|---|
| Rose | Esselink et al. (2003) | 4 | 50 | 24c | 5 | 3 | 6 |
| Süss and Schultze (2003) | 3 | 60 | 130d | 46 | 36 | 40 | |
| Zhang et al. (2006) | 1, 2 or 3b | 50–58b | 22e | 6 | 1 | 0 | |
| Hibrand Saint Oyant et al. (2008) | 4 | 55 | 21f | 3 | 7 | 2 | |
| Strawberry | Hadonou et al. (2004); Sargent et al. (2004, 2006) | 4 | 50 | 28g | 21 | 6 | 1 |
| Lewers et al. (2005); Cipriani et al. (2006) | 4 | 50 | 24h | 21 | 1 | 1 | |
| Peach | Rajapakse et al. (2001) | 1 | 55 Touch down | 4i | 2 | 2 | 0 |
| Dirlewanger et al. (2002) | 4 | 50 | 11j | 7 | 0 | 4 | |
| Apple | Rajapakse et al. (2001); Liebhard et al. (2002) | 1 | 55 | 2k | 0 | 1 | 0 |
aComposition of the reaction mixtures for the PCR amplification protocols described in Supplementary Material 1
bMore details are available upon request to the authors
cRhAB1, RhAB13, RhAB15, RhAB22, RhAB26, RhAB40, RhB19, RhB303, RhBK4, RhD201, RhD206, RhD221, RhE2b, RhE3, RhEO506, RhI402, RhJ404, RhL47, RhM405, RhO517, RhP507, RhP518, RhP519, RhP524
dRMS001-RMS055, RMS057, RMS058, RMS060-RMS110, RMS112-RMS116, RMS118, RMS119, RMS121, RMS123-RMS126, RMS128-RMS134, RMS137, RMS141, RMS144, RMS145
eRw3K19, Rw3N19, Rw4E22, Rw5D11, Rw8B8, Rw14H21, Rw10J19, Rw10M24, Rw17I7, Rw18N19, Rw22A3, Rw22B6, Rw23H5, Rw27A11B, Rw29B1, Rw32D19, Rw45E24, Rw46O8, Rw48N6, Rw55C6, Rw61F2, Rw62C4
fCL2845, CL2980, Contig172, CTG21, CTG329, CTG623, H10D03, H17C12, H24D11, H2F12, Rw15D15, Rw16E19, Rw20l17, Rw23F13, Rw25J16, Rw32K24, Rw34L6, Rw52D4, Rw53O21, Rw55E12, Rw59A12
gEMFn018, EMFn049, EMFn110, EMFn119, EMFn121, EMFn123, EMFn136, EMFn153, EMFn160, EMFn181, EMFn202, EMFn207, EMFn213, EMFn228, EMFn235, EMFv006, EMFv016, EMFv021, EMFv023, EMFv029, EMFv104, EMFv164, EMFvi008, EMFvi018, EMFvi025, EMFvi072, EMFvi108, EMFvi136
hARSFL_2, ARSFL_7, ARSFL_11, ARSFL_12, ARSFL_15, ARSFL_17, ARSFL_18, ARSFL_22, ARSFL_24, ARSFL_27, ARSFL_28, ARSFL_31, ARSFL_92, ARSFL_96, Fvi-11, UDF-002, UDF-006, UDF-016, UDF-018, UDF-019, UDF-025, UDF-033, UDF-055, UDF-065, UDF-0
iPchgms3, Pchgms41, Pchcms2, Pchgms2
jBPPCT008, BPPCT013, BPPCT014, BPPCT017, BPPCT030, BPPCT031, BPPCT035, BPPCT037-BPPCT039, BPPCT041
k01a6, CH02C11