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Sema3c and VEGF165 stimulate endothelial cell tube formation and sema3a inhibits tube formation. Mouse glomerular endothelial cells plated on collagen I gels, serum free, for 48 h and labeled with Cell Tracker®: a Control endothelial cell network; b sema3c (300 ng/ml) induces extensive capillary-like network formation; c VEGF165 (30 ng/ml) induces capillary-like network formation; d sema3a (250 ng/ml) abrogates most of endothelial cell tube formation
