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. Author manuscript; available in PMC: 2013 Jul 20.
Published in final edited form as: Vaccine. 2012 Jun 9;30(34):5099–5109. doi: 10.1016/j.vaccine.2012.05.067

Figure 3. Comparison of pooled rabbit sera from non-immune animals versus animals hyperimmune to TSST-1 mutants G31S/S32P, H135A, and Q136A to inhibit superantigenicity of TSST-1 (1 μg/well), as tested in a 4 day assay with rabbit splenocytes.

Figure 3

Splenocytes were incubated with designated dilutions of sera + TSST-1 for 3 days, and then 1 μCi 3H-thymidine added for 24 hr. DNA was harvested and counts/min determined as a measure of lymphocyte proliferation. Counts/min splenocytes + TSST-1 = 110,801 ± 8647. Counts/min splenocytes alone = 7248 ± 1164.