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. Author manuscript; available in PMC: 2013 Jul 20.
Published in final edited form as: Vaccine. 2012 Jun 9;30(34):5099–5109. doi: 10.1016/j.vaccine.2012.05.067

Figure 6. Wild-type TSST-1 immobilized on beads pulls-down CD40.

Figure 6

TSST-1 was immobilized on magnetic Dynabeads and then used to bind purified CD40. Control beads lacked TSST-1 and were treated comparably with CD40. After washing, beads were treated with SDS-PAGE buffer and samples electrophoresed in SDS-PAGE gels. Subsequently, proteins were transferred to PVDF membranes and probed with monoclonal antibodies to CD40, alkaline phosphatase-conjugated antibodies to mouse immunoglobulin, and then substrate.