Fig. 3.

Oxidized LDLs do not affect surface expression of TLR2, TLR4, and their coreceptors CD14, CD11b, and CD36. Control (upper panel) or treated for 3 h with oxLDLs (lower panel) monocytes were stained with PE-conjugated antihuman TLR2, TLR4, CD36 mAbs, or FITC-conjugated antihuman CD14, CD11b mAbs and analyzed by flow cytometry. The histogram markers were set based on the signal from appropriate isotype controls, and they designate section of the histogram representing positive cells. The percent of positive cells and MFI values are given in histograms. Results of a representative experiment of three performed are presented.