Table 2.
Acid-Ceramidase Activity and Ceramide Content in Control and Farber Fibroblasts and in WT and Morphant Zebrafish Embryos
| Acid-Ceramidase Activity (pmol/h/mg) | Acid-Ceramidase Activity (%) | Ceramide Content (pmol/mg) | |
|---|---|---|---|
| Experiment 1 | |||
| Control fibroblasts | 8,556 | 958 | |
| Farber fibroblasts | 267 | 3.1% | 8,004 |
| Experiment 2 | |||
| WT zebrafish-1 | 5,172 | 1,123 | |
| MO zebrafish-1 | 1,324 | 25.6% | 637 |
| MO zebrafish-2 | 561 | 10.8% | 786 |
| Experiment 3 | |||
| WT zebrafish-2 | 7,319 | 885 | |
| MO zebrafish-3 | 3,064 | 41.9% | 786 |
Each determination was performed in duplicate. The residual acid-ceramidase activity (%) is shown as the ratio of mutant to WT activity within each set of experiments. The following abbreviations are used: WT, wild-type; and MO, morphant.