Ideograms of Balanced Chromosome Translocations and FISH Mapping of the Chromosomal Breakpoints
(A and B) Ideograms illustrating the t(11;19)(p11.2;p13.2)dn karyotype in DGAP012 and the t(1;11)(p21.1;p11.2)dn karyotype in MCN1762; this latter karyotype was revised from the original karyotypic assessment, t(1;11)(p13;p11)dn.
(C) FISH analysis with a 30 kb SnaBI restriction fragment (signal shown in green) from BAC CTD-3193O13, which has previously been reported as a breakpoint-crossing clone.24 Probe hybridization is seen on the normal 19, der(19), and der(11) chromosomes, indicating that this 30 kb restriction fragment spans the chromosome 19 breakpoint region (Figure S1B).
(D) FISH analysis with 37 kb fosmid G248P88722D5 spanning the breakpoints on the normal 11, der(1), and der(11) chromosomes, indicating that the translocation breakpoint of the chromosome 11 is located within the sequence of this genomic clone.