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Occlusion of pores. a) Agarose pore channels coated with capture antibody. b) Smaller bodies, such as IL-1β antigen (4.5 nm, 35 nm immunocomplex), allow penetration deeper into the bead interior than larger immunocomplexes, such as c) CEA (27 nm, 57 nm immunocomplex), which may block reagent transport in the agarose pores. d) At lower concentrations of capture antibody, occlusion is circumvented.
