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Complement activation by surface-bound SALSA. In an ELISA assay mannan (A) or rSALSA (B) were coated in microtiter wells. NHS, MBL-deficient serum, MgEGTA-serum, or HIS (all 10%) was added and C4 and C3 deposition was measured using specific antibodies. Averages and SDs of three experiments performed in duplicate are shown.
