FIGURE 7.

Complement inhibition by fluid-phase SALSA. In a flow cytometry assay C. albicans was incubated in 10% NHS, MBL-deficient serum, MgEGTA-serum, or HIS with rSALSA (0–15 μg/ml). Complement deposition was measured using anti-C4 (A) and anti-C3 (B) antibodies. In (A) an inhibition of C4 deposition was observed when SALSA was added to NHS. (B) shows a dose-dependent inhibition of C3 deposition from NHS. The results are expressed as percentage of MFI in the absence of SALSA. Averages and SDs of three separate experiments are shown.