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. 2012 Jun 27;142(8):1495–1503. doi: 10.3945/jn.112.158865

FIGURE 1.

FIGURE 1

The feed-deprived feeding response of hepatic nuclear SREBP1, ChREBP, and MLX (A) and hepatic lipogenic gene expression (B) in Ldlr−/− mice fed the HFHC-OO or HFHC-MO diets for 12 wk. (A) After 12 wk of being fed these diets, the mice were deprived of food overnight. At 0800 the next day, one-half of the mice in each group were fed their diets, i.e., HFHC-OO or HFHC-MO. Feed-deprived and fed mice were killed at 0800 and 1200, respectively, for the quantitation of hepatic nuclear SREBP1, ChREBP, and MLX. Mean (±SD) results are expressed as nuclear protein abundance, fold of feed-deprived HFHC-OO; n = 8. Data were analyzed by 2-way ANOVA. Labeled means without a common letter differ, P < 0.05. (B) Hepatic RNA from the fed mice described above was used to quantify Acc1, Fasn, Scd1, and Elovl6 mRNA by qRT-PCR. Mean (±SD) results are expressed as mRNA abundance, fold of HFHC-OO; n = 8. Data were analyzed by Student’s t test. Labeled means without a common letter differ, P < 0.05. ACC1, acetyl CoA carboxylase-1; ChREBP, carbohydrate response element binding protein; Elov16, fatty acid elongase-6; FASN, fatty acid synthase; HFHC-MO, high-fat–high-cholesterol with olive oil supplemented with menhadin oil; HFHC-OO, high-fat–high-cholesterol with olive oil; MLX, max-like protein X; SCD1, stearoyl CoA desaturase-1; SREBP-1, sterol regulatory element binding protein-1.