. 2012 Jun 27;142(8):1495–1503. doi: 10.3945/jn.112.158865

TABLE 1.

Phenotypic comparison of WT and Ldlr^−/− mice fed the NP, HF, HFHC-OO, or HFHC-MO diets for 12 wk¹

	WT				Ldlr^−/−
	NP²	HF	HFHC-OO	HFHC-MO	HFHC-OO	HFHC-MO
Body weight,³ g	28 ± 2^a	45 ± 4^b	43 ± 1^b	39 ± 2^b	40 ± 5^b	40 ± 5^b
Plasma variables⁴
Glucose, mmol/L	6.0 ± 1.0	9.3 ± 2.7	8.3 ± 2.8	5.6 ± 0.7	11.9 ± 4.6	11.0 ± 4.6
TG,⁵ ⁶ mg/dL	120 ± 44^a	90 ± 16^a	66 ± 27^a	106 ± 25^a	352 ± 141^b	185 ± 82^a
Cholesterol,³ ⁵ mg/dL	52 ± 21^a	108 ± 46^a	138 ± 66^b	81 ± 6^a	957 ± 252^b	599 ± 145^b
Apo B,⁵ ⁶ mg/dL	11 ± 2^a	14 ± 2^a	15 ± 4^a	14 ± 2^a	160 ± 70^b	50 ± 21^a
Apo CIII,⁵ ⁶ mg/dL	13 ± 3^a	13 ± 7^a	10 ± 4^a	8 ± 2^a	21 ± 6^b	11 ± 6^a
NEFA,⁵ ⁶ mEq/ml	0.8 ± 0.2^a	0.7 ± 0.2^a	0.6 ± 0.1^a	0.7 ± 0.3^a	1.5 ± 0.6^b	0.9 ± 0.4^a
β-Hydroxybutyrate,⁵ mmol/L	1.4 ± 0.3^a	1.3 ± 0.4^a	1.5 ± 0.3^a	1.7 ± 0.2^a	2.9 ± 0.8^b	2.6 ± 0.5^b
ALT,³ ⁵ ⁶ U/L	4 ± 1^a	19 ± 10^b	20 ± 11^b	8 ± 2^b	38 ± 9^c	21 ± 16^b,c
AST,³ ⁵ ⁶ U/L	10 ± 2^a	18 ± 2^a	30 ± 12^b	13 ± 2^a	56 ± 1^c	27 ± 12^b
Liver variables
Weight, g	1.0 ± 0.1	1.6 ± 0.5	1.9 ± 0.5	1.2 ± 0.1	2.0 ± 0.6	1.6 ± 0.4
% Body weight³	3.6 ± 0.1^a	3.4 ± 1.0^a	4.5 ± 1.2^b	3.0 ± 0.2^a	4.8 ± 0.9^b	3.9 ± 0.7^a
TG,³ ⁷ μg/mg	51 ± 23^a	157 ± 88^b	141 ± 63^b	72 ± 15^a	129 ± 13^b	64 ± 26^a
Cholesterol,³ ⁵ ⁶ ⁸ μg/mg	7 ± 1^a	6 ± 2^a	8 ± 1^a	8 ± 1^a	17 ± 3^b	10 ± 5^a

Values are means ± SD; = 8/treatment group. Labeled means in a row with superscripts without a common letter differ, P < 0.05. ALT, alanine aminotransferase; AST, aspartate aminotransferase; HF, high-fat; HFHC-MO, high-fat–high-cholesterol with olive oil supplemented with menhadin oil; HFHC-OO, high-fat–high-cholesterol with olive oil; NEFA, nonesterified fatty acid; NP, nonpurified; WT, wild-type.

NP ^−/− mice were used as the control group.

Two-way ANOVA diet effect, < 0.05.

⁴

To convert from mg/dL to g/L, multiply by 0.0113 for TG and 0.02586 for cholesterol. To convert from mg/dL to g/L, multiply by 0.01 for apo B and apo CIII. To convert from mEq/mL to mEq/L, multiply by 1000 for NEFA.

⁵

Two-way ANOVA genotype effect, < 0.05.

⁶

Two-way ANOVA diet × gene interaction effect, < 0.05.

⁷

Data are expressed as g TG/mg protein (liver); to convert from μg/mg to mmol/g, multiply by 0.00113.

⁸

Data are expressed as g cholesterol/mg protein (liver); to convert from μg/mg to mmol/g, multiply by 0.002586.