TABLE 2.

Hepatic fatty acid composition in Ldlr^−/− mice fed the NP, HFHC-OO, or HFHC-MO diets for 12 wk¹

	NP	HFHC-OO	HFHC-MO
Fatty acids, mol%
16:0	26.9 ± 1.0a	18.0 ± 1.9b	14.0 ± 4.7b
16:1(n-7)	2.2 ± 0.7	3.4 ± 0.9	2.2 ± 1.9
18:0	11.7 ± 2.7a	2.4 ± 0.5b	4.0 ± 2.3b
18:1(n-9)	15.2 ± 1.8a	55.0 ± 4.3b	37.6 ± 3.7c
18:1(n-7)	3.1 ± 0.3a	7.3 ± 1.2b	5.1 ± 1.1c
18:2(n-6)	24.7 ± 2.8a	14.2 ± 1.6b	16.4 ± 3.0b
18:3(n-3)	0.8 ± 0.2	0.3 ± 0.1	0.3 ± 0.1
20:0	0.4 ± 0.1	0.3 ± 0.1	0.6 ± 0.1
20:2(n-6)	0.2 ± 0.1a	1.8 ± 0.1b	0.8 ± 0.2a
20:3(n-6)	0.7 ± 0.2	0.6 ± 0.1	0.4 ± 0.1
20:4(n-6)	6.2 ± 0.1a	3.2 ± 0.7b	2.0 ± 1.1b
20:5(n-3)	0.6 ± 0.1a	0.2 ± 0.1a	7.9 ± 2.3b
22:5(n-6)	0.2 ± 0.1	0.8 ± 0.2	0.6 ± 0.2
22:5(n-3)	0.6 ± 0.1a	0.2 ± 0.1a	2.0 ± 0.3b
22:6(n-3)	5.1 ± 0.9a	1.4 ± 0.3b	9.6 ± 3.8c
Σ C20–222
C20–22(n-3)	6.3 ± 0.6a	1.8 ± 0.3b	19.5 ± 3.3c
C20–22(n-6)	7.3 ± 1.3a	6.4 ± 0.8a	3.7 ± 1.1b
Fatty acid ratios
20:4(n-6) to 18:2(n-6)	0.26 ± 0.07a	0.27 ± 0.08a	0.14 ± 0.09b
20:3(n-6) to 18:2(n-6)	0.04 ± 0.02a	0.05 ± 0.01a	0.02 ± 0.01b

¹Values are means ± SD and are representative of 2 separate studies; = 4/treatment group. Labeled means in a row with superscripts without a common letter differ, P < 0.05. HFHC-MO, high-fat–high-cholesterol with olive oil supplemented with menhadin oil; HFHC-OO, high-fat–high-cholesterol with olive oil; NP, nonpurified.

²Sum C20-22 refers to the sum of all the 20 to 22 carbon fatty acids analyzed.