TABLE 4.
Hepatic gene expression in Ldlr−/− mice fed the NP, HFHC-OO, or HFHC-MO diets for 12 wk1
| NP | HFHC-OO | HFHC-MO | |
| Oxidative stress | Fold of NP | ||
| Hmox1 | 1.0 ± 0.3 a | 4.2 ± 1.0 b | 3.2 ± 1.1 b |
| PUFA synthesis | |||
| Elovl2 | 1.0 ± 0.4a | 2.2 ± 0.9b | 2.4 ± 1.1b |
| Elovl5 | 1.0 ± 0.2 a | 0.8 ± 0.1a | 0.3 ± 0.1b |
| Fads1 | 1.0 ± 0.1 a | 0.8 ± 0.3a | 0.2 ± 0.1b |
| Fads2 | 1.0 ± 0.1 | 1.1 ± 0.2 | 0.7 ± 0.3 |
| Cholesterol metabolism | |||
| HMG CoA red | 1.0 ± 0.3 | 1.0 ± 0.2 | 1.2 ± 0.4 |
| HMG CoA syn 1 | 1.0 ± 0.2 | 1.0 ± 0.1 | 0.9 ± 0.2 |
| Cyp7A1 | 1.0 ± 0.3 | 1.2 ± 0.2 | 2.1 ± 1.3 |
| Abca1 | 1.0 ± 0.2 a | 4.7 ± 0.3b | 4.0 ± 0.4b |
| Abcg5 | 1.0 ± 0.1a | 3.2 ± 0.3b | 4.0 ± 0.5b |
| Acat1 | 1.0 ± 0.4a | 4.8 ± 1.5b | 3.0 ± 1.1c |
| Acat2 | 1.0 ± 0.1a | 2.0 ± 0.5b | 2.3 ± 0.7b |
| Inflammation | |||
| Mcp1 | 1.0 ± 0.5a | 22.8 ± 10.4b | 4.6 ± 2.8a |
| Cd68 | 1.0 ± 0.5a | 7.7 ± 2.5b | 4.1 ± 2.5c |
| Clec4f | 1.0 ± 0.5a | 6.3 ± 1.2b | 4.7 ± 1.3b |
| Fibrosis | |||
| Tgfβ1 | 1.0 ± 0.2a | 3.0 ± 0.9b | 2.1 ± 0.4b |
| Procol1α1 | 1.0 ± 0.7a | 43.0 ± 24.0b | 20.1 ± 14.0c |
1
Values are means ± SD and are fold of NP; = 8. Labeled means in a row with superscripts without a common letter differ, P < 0.05. HFHC-MO, high-fat–high-cholesterol with olive oil supplemented with menhadin oil; HFHC-OO, high-fat–high-cholesterol with olive oil; NP, nonpurified.