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. 2012 Jun 13;142(8):1582–1589. doi: 10.3945/jn.112.157883

TABLE 1.

Experimental diet fatty acid profiles1

Fatty acid SO FO
% of total fatty acids
14:0 <0.2 4.5
16:0 10.9 17.6
18:0 5.0 3.5
18:1 23.8 16.8
18:2(n-6) 52.8 12.7
18:3(n-3) 6.4 1.6
20:5(n-3) (EPA) 15.9
22:5(n-3) 2.9
22:6(n-3) (DHA) 12.0
1

Experimental diet formulations were based on the AIN-93G diet but with modifications to allow the incorporation of a higher amount of fat without changing the nutrient:energy ratio. Diets contained (g/kg): corn starch (375), casein (230), sucrose (110), fiber (α-cellulose) (57), AIN-93 mineral mix (40.2), AIN-93G vitamin mix (11.5), L-cysteine (3.4), choline bitartrate (2.9), and 1 of 2 fat sources (180). The dietary fat sources were either SO or a mixture of menhaden FO and SO (in a 4:1 ratio). Both fat sources were stabilized against auto-oxidation with the inclusion of 0.02% (wt:wt) tertiary butylhydroquinone. Fatty acids present in the FO diet at levels >1% of the total, but not listed above, include: 16:1(n-7) (7.1%), 18:1(n-7) (1.6%), 18:4(n-3) (2.7%), and 20:4(n-3) (1.0%). FO, fish oil; SO, soy oil.