TABLE 1.
Experimental diet fatty acid profiles1
| Fatty acid | SO | FO |
|
% of total fatty acids |
||
| 14:0 | <0.2 | 4.5 |
| 16:0 | 10.9 | 17.6 |
| 18:0 | 5.0 | 3.5 |
| 18:1 | 23.8 | 16.8 |
| 18:2(n-6) | 52.8 | 12.7 |
| 18:3(n-3) | 6.4 | 1.6 |
| 20:5(n-3) (EPA) | — | 15.9 |
| 22:5(n-3) | — | 2.9 |
| 22:6(n-3) (DHA) | — | 12.0 |
Experimental diet formulations were based on the AIN-93G diet but with modifications to allow the incorporation of a higher amount of fat without changing the nutrient:energy ratio. Diets contained (g/kg): corn starch (375), casein (230), sucrose (110), fiber (α-cellulose) (57), AIN-93 mineral mix (40.2), AIN-93G vitamin mix (11.5), L-cysteine (3.4), choline bitartrate (2.9), and 1 of 2 fat sources (180). The dietary fat sources were either SO or a mixture of menhaden FO and SO (in a 4:1 ratio). Both fat sources were stabilized against auto-oxidation with the inclusion of 0.02% (wt:wt) tertiary butylhydroquinone. Fatty acids present in the FO diet at levels >1% of the total, but not listed above, include: 16:1(n-7) (7.1%), 18:1(n-7) (1.6%), 18:4(n-3) (2.7%), and 20:4(n-3) (1.0%). FO, fish oil; SO, soy oil.