Table 1.
Tomato chlorotic dwarf viroid (TCDVd) sequence variant population structure, occurrence frequency and nucleotide variation in TCDVd isolate Trust-infected tomato plants.
Sequence variant | Size (nt) | Occurrence frequency (%) | Nucleotide difference from the reference sequence a | |
---|---|---|---|---|
Plant 1 | Plant 2 | |||
AF162131 | 360 | n/a | n/a | n/a |
I | 360 | 66.7 | 65.7 | No variation |
II | 360 | 33.3 | 28.6 | 178 (U to A); 182 (C to U); 183 (A to U) |
III | 360 | 0 | 2.9 | 165 (U to C) |
IV | 360 | 0 | 2.9 | 149 (A to G) |
Haplotype diversity b | 0.500 ± 0.128 | 0.499 ± 0.071 | ||
Nucleotide diversity c | 0.00417 ± 0.00107 | 0.00382 ± 0.00057 |
a AF162131 was used as a reference sequence. Nucleotide differences in each variant were indicated in relation to the reference sequence at positions in the reference; b/c Haplotype and nucleotide diversities were calculated using the software DnaSP v5; n/a: not applicable.