Skip to main content
. Author manuscript; available in PMC: 2012 Aug 1.
Published in final edited form as: Cryobiology. 2011 Apr 28;63(1):46–55. doi: 10.1016/j.cryobiol.2011.04.008

Figure 1. Viability Assessment of Hypothermically Stored Human Corneal Endothelial Cells.

Figure 1

HCEC’s were placed at 4°C in either: Complete Media (CGM), HBSS with Ca++ and Mg++, ViaSpan, or OptiSol for 18 hours, 1 day, or 2 days. Resultant cell viability following storage was determined 0, 24 and 48 hours post-storage. The data illustrate profound differences between storage media and sensitivity of corneal endothelial cells to cold exposure in vitro. Immediate following 18 hours of storage viabilities of 14.9% (±3.8), 99.0% (±8.2), 91.5% (±4.0), and 57.4% (±6.5) were noted for CGM, HBSS, ViaSpan and OptiSol storage, respectively. As the interval was increased to 2 days, only ViaSpan samples remained viable (68.2% ±7.7).